Aktuálně řešené grantové projekty

GAČR - Mezidruhový přenos drůbežích testikulárních buněk
GAČR - Vektory odvozené z ptačích sarkomových a leukozových virů a jejich potenciál pro transgenesi u drůbeže

MŠMT - Spermatogoniální kmenové buňky v procesu kuřecí spermatogeneze
MŠMT - Moderní biotechnologické postupy umožňující dlouhodobé uchování drůbeží genetické informace

Patenty

Český patent CZ 289464: Způsob konstrukce transgenní drůbeže
Evropský patent č. 1211932: A method of transgenic fowl construction
Český patent CZ 293226: Způsob přípravy expresních vektorů k produkci terapeutických proteinů v transgenních zvířatech.
Evropský patent EP 1217072: Method of the preparation of an expression vector for the production of the therapeutic proteins in the transgenic animals.

Publikace

Vrba V, Blake DP, Poplstein M. (2010): Quantitative real-time PCR assays for detection and quantification of all seven Eimeria species that infect the chicken. Vet Parasitol 174(3-4):183-190

Trefil P, Bakst MR, Yan H, Hejnar J, Kalina J, Mucksova J. (2010): Restoration of spermatogenensis after transplantation of c-Kit positive testicular cells in the fowl. Theriogenology 74(9):1670-1676

Mucksova J, Brillard J-P, Hejnar J, Poplstein M, Kalina J, Bakst M, Yan H, Trefil P. (2009): Identification of various testicular cell populations in pubertal and adult cockerels. Animal Reproduction Science 114(4):415-422

Pakandl M, Hlásková L, Poplštein M, Chromá V, Vodička T, Salát J, Mucksová J. (2008): Dependence of the immune response to coccidiosis on the age of rabbit suckling. Parasitol Res 103(6):1265-1271

Pakandl M, Hlásková L, Poplštein M, Nevečeřalová M, Vodička T, Salát J, Mucksová J. (2008): Immune response to rabbit coccidiosis: a comparison between infections with Eimeria flavescens and Eimeria intestinalis. Folia Parasitol 55: 1-6

Kalina J, Senigl F, Micakova A, Mucksova J, Blazkova J, Yan H, Poplstein M, Hejnar J, Trefil P. (2007): Retrovirus-mediated in vitro gene transfer into chicken male germ line cells. Reproduction 134(3): 445-453

Trefil P, Micakova A, Mucksova J, Hejnar J, Poplstein M, Bakst MR, Kalina J, Brillard J-P. (2006): Restoration of spermatogenesis and male fertility by transplantation of dispersed testicular cells in the chicken. Biology of Reproduction 75(4):575-581

Markova J, Smetana K, Jenikova G, Lachova J, Krejcirikova V, Poplstein M, Fabry M, Brynda J, Alvarez RA, Cummings RD, Maly P. (2006): Role of carbohydrate recognition domains of mouse galectin-4 in oligosaccharide binding and epitope recognition and expression of galectin-4 and galectin-6 in mouse cells and tissues. Int. J. Mol. Med. 18: 65-76

Yan H, Trefil P, Bingnan X, Shixun L, Wu X-L. (2004): Transfection of blastodermal cell sof Heifeng Silkie chicken using GFP plasmid. Advance on Animal Genetic and Breeding Researches in China, 469-473.

Yan H, Bingnan X, Trefil P, Gio D-X, Wu X-L. (2004): Achievements and Applications in making chicken chimeras using BCs. Hereditas (Beijing) 26(4): 537-543.

Trefil P, Mičáková A, Mucksová J, Poplštein M, Brillard J-P, Hodek P. (2004): Sex reversed chicks (Gallus domesticus) hatched from eggs treated with aromatase inhibitor YM 511. Czech. J. Anim. Sci., 49 (12): 511-516.

Mikuš T, Poplštein M, Sedláková J, Landa V, Jeníková G, Trefil P, Lidický J. Malý P. (2004): Generation and phenotypic analysis of a transgenic line of rabbits secreting active recombinant human erythropoietin in the milk. Transgenic Research, 13: 487-498.

Trefil P, Mičáková A, Stiborová M, Poplštein M, Brillard J-P, Hodek P. (2004): Effects of alfa-naphtoflavone on body growth and gonad development in chickens (Gallus domesticus). Czech J.Anim.Sci., 49 (6):231-238.

Pakandl M, Černík F, Coudert P. (2003): The rabbit coccidium Eimeria flavescens Marotel and Guilhon, 1941: an electron microscopic study of its life cycle. Parasitol. Res. 91: 304-311

Mičáková A, Hodek P, Poplštein M, Trefil P. (2003): Application of α-naftoflavone during the growth and development of broilers. Chem. Listy, 97,155-156.

Yan H, Bingnan X, Trefil P, Shixun L. (2003): Progresses on Preservation of Poultry Genetic Resources. Preservation of Resources, 1008-0414:21-23.

Trefil P, Polák J, Poplštein M, Mikuš T, Kotrbová A, Rozinek J. (2003): Preparation of fowl tetstes as recipient organs to germ-line chimeras by means of gamma-radiation. British Poultry Science. Vol.44, 4: 643-650.

Kalina J, Kolmanová A, Mikuš T, Mičáková A, Trefil P. (2003): Transfection of cock spermatogonial cells via electroporation and lipofection. Czech J.Anim.Sci. 48(7):279-84