Projects, patents and publications

Current grant projects

TACR - DNA microchip for the direct diagnosis of pathogenic bacteria.
TACR - Diagnostic kit for the detection of chemical damage of the environment based on cell cultures.
MZe ČR - Advancement of chicken transgenic technology and its biotechnological application
GAČR - Transgenic models of transposon insertional mutagenesis in domestic chicken

TACR - Genetic program for 2 lines hybrid of layers (breed) for egg production and cockerel meet production in Vietnam and South Asia farming condition.
GACR - Gene targeting on chicken and the resistance to newly emerging retroviruses.
TACR - The life attenuated vaccine complemented with recombinant antigens against Salmonella and coccidiosis.
GAČR – Interspecies transfer of poultry testicular cells
GAČR - Vectors derived from avian sarcoma and leucosis virus and their potential for poultry transgenesis
MŠMT - Spermatogonial Stem Cells in the Process of Chicken Spermatogenesis
MŠMT - Modern bio-techniques procedures allowing long term conservation of poultry cells


Czech patent CZ 289464: Method of transgenic fowl construction
European patent No. 958098.6: A Method of transgenic fowl construction
Czech patent CZ 293226: Method of preparation of express vectors for the production of transgenic animals.
European patent No. 1217072: Method of the preparation of an expression vector for the production of the therapeutic proteins in the transgenic animals.


Mucksová J, Reinišová M, Kalina J, Lejčková B, Hejnar J, Trefil P. (in press): Conservation of chicken male germ line by orthotopic transplantation of primordial germ cells from genetically distant donors. Biology of Reproduction

Mucksová J, Chalupský K, Plachý J, Kalina J, Rachačová P, Staněk O, Trefil P. (2018): Simultaneous detection of chicken cytokines in plasma samples using the Bio-Plex assay. Poultry Science 97(4):1127-1133

Trefil P, Aumann D, Koslová A, Mucksová J, Benešová B, Kalina J, Wurmser C, Fries R, Elleder D, Schusser B, Hejnar J. (2017): Male fertility restored by transplanting primordial germ cells into testes: a new way towards efficient transgenesis in chicken. Scientific Reports 7, 14246

Mucksová J, Plachý J, Staněk O, Hejnar J, Kalina J, Benešová B, Trefil P. (2017): Cytokine response to the RSV antigen delivered by dendritic cell‑directed vaccination in congenic chicken lines. Veterinary Research 48:18

Mucksová J, Plachý J, Staněk O, Hejnar J, Kalina J, Benešová B, Trefil P. (In press): Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines. Veterinary Research

Benešová B, Trefil P. (2016): Possibilities for preserving genetic resources in birds. World's Poultry Science Journal 72(3): 629-942

Vrba V, Pakandl M. (2015): Host specificity of turkey and chicken Eimeria: controlled cross-transmission studies and a phylogenetic view. Vet Parasitol 208(3-4):118-124

Benešová B, Mucksová J, Kalina J, Trefil P. (2014): Restoration of spermatogenesis in infertile male chickens after transplantation of cryopreserved testicular cells. British Poultry Science 55(6):837-845

Vrba V, Pakandl M. (2014): Coccidia of turkey: from isolation, characterisation and comparison to molecular phylogeny and molecular diagnostics.Int J Parasitol 44(13):985-1000

Mucksová J, Kalina J, Bakst M, Yan H, Brillard JP, Benešová B, Fafílek B, Hejnar J, Trefil P. (2013). Expression of the chicken GDNF family receptor alfa-1 as a marker of spermatogonial stem cells. Animal Reproduction Science 142(1-2):75-83

Kalina J, Mucksová J, Yan H, Trefil P. (2012): Rapid sexing of selected Galliformes by polymerase chain reaction. Czech J Anim Sci 57(4):187–192

Trefil P, Mucksová J, Kalina J. (2012): Chicken Testicular Stem Cells. The Journal of Poultry Science 49(3):150-154

Vrba V, Poplštein M, Pakandl M. (2011): The discovery of the two types of small subunit ribosomal RNA gene in Eimeria mitis contests the existence of E. mivati as an independent species. Vet Parasitol 183(1-2):47-53

Vrba V, Blake DP, Poplstein M. (2010): Quantitative real-time PCR assays for detection and quantification of all seven Eimeria species that infect the chicken. Vet Parasitol 174(3-4):183-190

Trefil P, Bakst MR, Yan H, Hejnar J, Kalina J, Mucksova J. (2010): Restoration of spermatogenensis after transplantation of c-Kit positive testicular cells in the fowl. Theriogenology 74(9):1670-1676

Mucksova J, Brillard J-P, Hejnar J, Poplstein M, Kalina J, Bakst M, Yan H, Trefil P. (2009): Identification of various testicular cell populations in pubertal and adult cockerels. Animal Reproduction Science 114(4):415-422

Pakandl M, Hlásková L, Poplštein M, Chromá V, Vodička T, Salát J, Mucksová J. (2008): Dependence of the immune response to coccidiosis on the age of rabbit suckling. Parasitol Res 103(6):1265-1271

Pakandl M, Hlásková L, Poplštein M, Nevečeřalová M, Vodička T, Salát J, Mucksová J. (2008): Immune response to rabbit coccidiosis: a comparison between infections with Eimeria flavescens and Eimeria intestinalis. Folia Parasitol 55: 1-6

Kalina J, Senigl F, Micakova A, Mucksova J, Blazkova J, Yan H, Poplstein M, Hejnar J, Trefil P. (2007): Retrovirus-mediated in vitro gene transfer into chicken male germ line cells. Reproduction 134(3): 445-453

Trefil P, Micakova A, Mucksova J, Hejnar J, Poplstein M, Bakst MR, Kalina J, Brillard J-P. (2006): Restoration of spermatogenesis and male fertility by transplantation of dispersed testicular cells in the chicken. Biology of Reproduction 75(4):575-581

Markova J, Smetana K, Jenikova G, Lachova J, Krejcirikova V, Poplstein M, Fabry M, Brynda J, Alvarez RA, Cummings RD, Maly P. (2006): Role of carbohydrate recognition domains of mouse galectin-4 in oligosaccharide binding and epitope recognition and expression of galectin-4 and galectin-6 in mouse cells and tissues. Int. J. Mol. Med. 18: 65-76

Yan H, Trefil P, Bingnan X, Shixun L, Wu X-L. (2004): Transfection of blastodermal cell sof Heifeng Silkie chicken using GFP plasmid. Advance on Animal Genetic and Breeding Researches in China, 469-473.

Yan H, Bingnan X, Trefil P, Gio D-X, Wu X-L. (2004): Achievements and Applications in making chicken chimeras using BCs. Hereditas (Beijing) 26(4): 537-543.

Trefil P, Mičáková A, Mucksová J, Poplštein M, Brillard J-P, Hodek P. (2004): Sex reversed chicks (Gallus domesticus) hatched from eggs treated with aromatase inhibitor YM 511. Czech. J. Anim. Sci., 49 (12): 511-516.

Mikuš T, Poplštein M, Sedláková J, Landa V, Jeníková G, Trefil P, Lidický J. Malý P. (2004): Generation and phenotypic analysis of a transgenic line of rabbits secreting active recombinant human erythropoietin in the milk. Transgenic Research, 13: 487-498.

Trefil P, Mičáková A, Stiborová M, Poplštein M, Brillard J-P, Hodek P. (2004): Effects of alfa-naphtoflavone on body growth and gonad development in chickens (Gallus domesticus). Czech J.Anim.Sci., 49 (6):231-238.

Pakandl M, Černík F, Coudert P. (2003): The rabbit coccidium Eimeria flavescens Marotel and Guilhon, 1941: an electron microscopic study of its life cycle. Parasitol. Res. 91: 304-311

Mičáková A, Hodek P, Poplštein M, Trefil P. (2003): Application of ?-naftoflavone during the growth and development of broilers. Chem. Listy, 97,155-156.

Yan H, Bingnan X, Trefil P, Shixun L. (2003): Progresses on Preservation of Poultry Genetic Resources. Preservation of Resources, 1008-0414:21-23.

Trefil P, Polák J, Poplštein M, Mikuš T, Kotrbová A, Rozinek J. (2003): Preparation of fowl tetstes as recipient organs to germ-line chimeras by means of gamma-radiation. British Poultry Science. Vol.44, 4: 643-650.

Kalina J, Kolmanová A, Mikuš T, Mičáková A, Trefil P. (2003): Transfection of cock spermatogonial cells via electroporation and lipofection. Czech J.Anim.Sci. 48(7):279-84